Mar 9 – 11, 2015
NH Laguna Palace Hotel
Europe/Rome timezone

Surface reactivity of CuO NPs is responsible for the early oxidative damages to A549 cells: a Trojan-horse independent mechanism

Mar 9, 2015, 5:30 PM
30m
Breakout 1 (NH Laguna Palace Hotel)

Breakout 1

NH Laguna Palace Hotel

Viale Ancona, n° 2 30172 Venice-Mestre, Italy Tel: +39 041 829 6111 Fax: +39 848 390 230
Parallel session 2A: Toxicology and human health risks 2A Toxicology and human health risks

Speaker

Elisa Moschini (University of Milano Bicocca)

Description

"Abstract: Background It has been demonstrated that CuO NPs are highly cytotoxic for the most of mammalian cells. The classical Trojan horse mechanism is retained to be the driver of cell death mainly after long exposure periods. This work aims to demonstrate that CuO NPs may have specific cell reactivity in the first phases of exposure independent from intracellular ion dissolution. Methods CuO NPs with similar primary size but different crystallinity and extracellular ROS production (Perelshtein et al., 2014) were administered to A549 cells as model for human toxicity. After assessing of cell viability SH- oxidation and protein carbonylation were monitored by immunocytochemistry and immunoblotting. Electron microscopy techniques were used to investigate cell-particle interactions. Results All NPs induced very early oxidative stress leading to a significant cell viability decrease after 3-6h of exposure. This effect was more pronounced for semi-crystalline CuO NPs and was independent from extra- and intra-cellular copper release although particles were detected both on cell surface and in cytoplasm already after 1h of exposure. Conclusions CuO NPs induce very early cell oxidative responses related to the specific NP surface reactivity, with semi-crystalline CuO NPs displaying the higher cytotoxicity. Since CuO-based NMs have been suggested as powerful biocidals a better characterization of the reactions at the bio-interfaces may help nanotechnologist in the safe-by-design synthesis of new antimicrobials."

Primary author

Elisa Moschini (University of Milano Bicocca)

Co-author

Paride Mantecca (University of Milano Bicocca)

Presentation materials