Roberta Ruotolo (Department of Life Sciences, University of Parma)
Nanotechnology is an emerging branch of applied science and technology for designing tools and devices at the nanoscale size (1-100 nm). Engineered nanomaterials (ENMs) have been widely used in fields such as electronics, medicine, physics, chemistry, biology, but also in the food and cosmetic industries. Little is known about the molecular mechanisms of cellular uptake and biological interactions with the ENMs. Adsorption of biomolecules to nanomaterials may influence cellular uptake, inflammation, accumulation, degradation and clearance of the ENMs. Understanding such relations is crucial for generating bio-compatible nanomaterials with controlled surface characteristics in a biological environment or for ENM-targeted delivery. The aim of this work was to study the biological effects of cadmium sulfide quantum dots (CdS QDs) in different cellular models. Integrating omic approaches such as transcriptomics, proteomics and phenomics, we identify new biological pathways important for CdS QD stress tolerance in yeast (Saccharomyces cerevisiae), plant (Arabidopsis thaliana) and human tumor cell lines. We have also investigated the capability of CdS QDs to adsorb human blood plasma or cell lysate proteins (yeast or tumor cell proteins) with two-dimensional gel electrophoresis and mass spectrometry identification (MALDI-TOF). In conclusion, our results provide new insights into the mechanisms of toxicity of metal-based nanomaterials.
Nelson Marmiroli (Department of Life Sciences, University of Parma)