Mar 9 – 11, 2015
NH Laguna Palace Hotel
Europe/Rome timezone

Development of a robust method for measuring engineered nanomaterial toxicity and uptake using Caenorhabditis elegans

Mar 10, 2015, 3:28 PM
24m
Breakout 2 (NH Laguna Palace Hotel)

Breakout 2

NH Laguna Palace Hotel

Viale Ancona, n° 2 30172 Venice-Mestre, Italy Tel: +39 041 829 6111 Fax: +39 848 390 230
Parallel session 4B: Toxicology and human health risks 4B Toxicology and human health risks

Speaker

Elijiah Petersen (NIST)

Description

Design and development of reliable cell-based nanotoxicology assays are important for evaluation of potentially hazardous engineered nanomaterials. Challenges to producing a reliable assay protocol include working with nanoparticle dispersions and living cell lines, and the potential for nano-related interference effects. We demonstrate the use of a 96-well plate design for a nano-cytotoxicity MTS cell viability assay. A detailed protocol and an inter-laboratory comparison are used to illustrate the variability of the assay with NH2-polystyrene nanoparticles. Data on both the within and between laboratory system controls can be used to evaluate the largest sources of variability in the protocol. This study suggests that a high level of agreement between each of the laboratories can be achieved, but consideration of protocol details such as cell line ID, cell rinsing, media removal, and nanoparticle dispersion is critical to ensure comparability of nanocytotoxicity assays results

Primary author

Co-authors

Presentation materials