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Mar 9 – 11, 2015
NH Laguna Palace Hotel
Europe/Rome timezone

The relationship between the biological effects of Titanium Dioxide nanofibers and their aspect ratio

Mar 11, 2015, 5:36 PM
24m
Breakout 1 (NH Laguna Palace Hotel)

Breakout 1

NH Laguna Palace Hotel

Viale Ancona, n° 2 30172 Venice-Mestre, Italy Tel: +39 041 829 6111 Fax: +39 848 390 230
Parallel Session 6A:Toxicology and human health risks 6AToxicology and human health risks

Speaker

Manfredi Allegri (Dept. of Biomedical, Biotechnological and Translational Sciences (SBiBiT), University of Parma)

Description

A strict relationship between the toxicity of fiber-like nanomaterials and their aspect ratio emerges from the fiber paradigm. As a consequence, fiber shortening is expected to reduce material toxicity. Titanium dioxide nanofibers (TiO2NF) are a novel fibrous nanomaterial, used in several industrial applications but still requiring complete toxicological characterization. We evaluated the toxicity of commercial TiO2NF (length, 0.2-30um; thickness, 0.2 to 0.6um; aspect ratio 1:28, consisting of primary TiO2 nanoparticles), before and after ball-milling, which lowered their aspect ratio to 1:8. The evaluated endpoints were cell viability, inflammatory markers, and trans-epithelial electrical resistance (TEER), an indicator of the epithelial barrier competence. TiO2 NF exhibited cell specific cytotoxicity, markedly decreasing viability in A549 epithelial cells but not in Raw 264.7 macrophages. A dose- and time-dependent TEER decrease in CaLu-3 cell monolayers was also detected. Ball-milling significantly mitigated these effects but, conversely, enhanced the expression of inflammatory markers in macrophages. This study indicates that TiO2NF exert significant toxic effects including cytotoxicity, macrophage activation and epithelial barrier impairment. While aspect ratio reduction mitigates TiO2NF effects on cell viability and epithelial barriers, it enhances the inflammogenic activity of the nanomaterial, indicating that different structural determinants are implied in the biological effects of fiber-like nanomaterials.

Primary author

Manfredi Allegri (Dept. of Biomedical, Biotechnological and Translational Sciences (SBiBiT), University of Parma)

Co-authors

Enrico Bergamaschi (Dept. of Clinical and Experimental Medicine, University of Parma) Massimiliano Bianchi (Dept. of Clinical and Experimental Medicine, University of Parma)

Presentation materials

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